Hundreds of genes. Thousands of samples. One solution.™
Plexigen’s geneCube™ platform is ideal for gene expression studies involving a small to medium number of genes in
a large number of samples. The platform has also been used for assaying a number of antibodies against a large number
of antigens, equivalent to a parallel ELISA and for SNP genotyping if a small to medium number of SNPs and large number
of samples are involved in an experiment.
Services are offered through the following two channels:
1. Direct service from Plexigen: A turn-key solution from experimental design to data analysis and a true customized service.
2. Service through Plexigen’s Marketing Collaborators: Plexigen is installing the geneCube platform at a number of qualified microarray service companies. Researchers can use the companies to process their samples.
Gene Expression Experiments Using geneCube Platform
Plexigen's geneCube platform is infinitely flexible for experimental design, which can meet customer's needs in gene content and accuracy requirements. A geneCube is essentially a deck of cards with each card coated with one or a number of oligo probes. The deck can be formed according to customer's gene content needs. Based on our extensive study, geneCube data with a total of four replications for each gene by sample combination can detect a 1.3 fold gene expression change in a wide range of gene expression levels. Customers can choose a number of replications for their experiments according to accuracy requirements.
Step |
Tasks |
Comments |
| 1 | Experimental design including determining number of replications in terms of geneCards and hybridizations and positive and negative controls according to customer’s specific needs | geneCube is infinitely flexible for experimental design |
| 2 | Design unique probe of oligo 50mers for each gene and probes have uniform hybridization condition | Using Plexigen’s proprietary algorithms and software. Avoid 2nd structures and repeats. Bias toward 3’ end. |
| 3 | Coat geneCards with high quality oligo probes and QC. Form geneCubes | |
| 4 | Sample preparation including RNA extraction, amplification and labeling | Customer can choose to do this step |
| 5 | Hybridization | |
| 6 | geneCard reading | |
| 7 | Statistical data analysis for identifying gene expression patterns | Plexigen will deliver customer signal intensity data and basic QC statistics if no additional statistical analysis is requested |
| 8 | Cross platform validation using RT-PCR | Available for extra cost |
Pricing
Setup fee: $10,000 (waived for number of samples > 200)
Covers experimental design, probe design, and oligo probe synthesis.
Per sample cost:
The price includes two geneCard replications and two feature replications.
|
Number of samples |
No RNA preparation |
With RNA preparation |
Per replication cost for additional replications |
|
<50 |
call for quote |
||
|
50 - 100 |
$350 |
$650 |
$60 |
|
101 - 500 |
$250 |
$500 |
$50 |
|
501 - 2000 |
$200 |
$450 |
$40 |
|
> 2000 |
call for quote |
Statistical Data Analysis
Our data analysis service provided software includes basic data analysis, such as repeatability analysis and analysis of variance.
For downstream analysis, please contact us.
Partial Data Validation
Plexigen guarantees an acceptable internal repeatability for geneCube replications. For cross platform validation, please contact us.
See our technology page for some examples of up to 10,000 samples, or follow this link to download a whitepaper describing Plexigen's gene expresion capabilities and results from current experiments. Plexigen_Gene_Expression.pdf (0.5 MB)
Genotyping Service
Follow this link to download a whitepaper describing Plexigen's genotyping capabilities. Plexigen_Genotyping.pdf (0.5 MB)
For pricing on genotyping please contact us.
Protein Interaction Assay Service
The geneCube™ can be used for detection of protein-protein and protein-compound interactions. For example, the geneCube can be used for parallel ELISA (Enzyme-linked immunosorbent assay) types of assays. For ELISA, an enzyme is linked chemically to a antibody or antigen. The unlabeled antigen or antibody is immobilized on a solid support, such as the bottom of a microtiter plate. For example, an antigen being immobilized on a solid support can assay a labeled antibody. The labeled antibody binds to the antigen, under the conditions where non-specific bindings are blocked. Antibody binding is detected by a reaction that coverts a colorless substrate into a colored reaction product. The ELISA type assays can be implemented on the geneCube platform in a parallel fashion. For example, 50 different antibodies can be coated on geneCards and assayed for 384 different antigens in parallel. One such cube will be equivalent to 4 X 50 = 200 microtiter plates. Besides high throughput, the geneCube platform requires a much smaller amount of sample solution for detection of 50 antigens.
Follow this link to download a whitepaper describing Plexigen's proteomic capabilities. Plexigen_Protein.pdf (0.5 MB)
For pricing on protein assay please contact us.
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Plexigen is seeking marketing collaborators in the following three areas:
- Gene expression service
- Protein interaction, including antibody and antigen detection service
- Genotyping service
Gene Expression Service
Plexigen is actively seeking marketing collaborators to split the tasks as a gene expression service provider.
| Tasks | |
| Plexigen | geneCard manufacture and quality control |
| Instrument manufacture, installation, and maintenance | |
| Provide information flow software | |
| Oligo probe design and synthesis | |
| Coating geneCards with oligo probes and quality control | |
| Design customer’s experiment in terms of replications, negative and positive controls, and geneCube arrangement | |
| geneCubes assembly and quality control | |
| Ship geneCubes to marketing collaborators | |
| Train technicians and provide technical assistance | |
| Marketing Collaborator | Do all necessary customer sample handling |
| Hybridize labeled RNA samples to geneCube | |
| Read the geneCubes | |
| Transfer data or final results to customers | |
| Customer | Provide samples |
Genotyping Service
Plexigen is actively seeking marketing collaborators to split the tasks as a genotyping service provider.
Protein Interaction Service
Plexigen is actively seeking marketing collaborators to split the tasks as a protein interaction service provider.
Current Marketing Collaborators
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